Coding

Part:BBa_K3807017:Experience

Designed by: Viktoriia Belousova   Group: iGEM21_KU_Leuven   (2021-10-01)


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Applications of BBa_K3807017

sfGFP was fused to a TetA gene through a flexible linker (encoded by GGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATTC). sfGFP serves as a reporter, whose expression level is measured by a spectrophotometer or flow-cytometer, for the expression level of TetA. The quantitative measurement of protein expression level was used to report the characteristics of theophylline riboswitches put directly upstream of this TetA-sfGFP gene.

User Reviews

UNIQ9906b988a1506683-partinfo-00000000-QINU UNIQ9906b988a1506683-partinfo-00000001-QINU